CryoEM structure of human gamma-secretase in complex with E2012 and L685458
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7d8x
Q-score: 0.603
Yang G, Zhou R, Guo X, Yan C, Lei J, Shi Y
Cell (2021) 184 pp. 521-533.e14 [ DOI: doi:10.1016/j.cell.2020.11.049 Pubmed: 33373587 ]
- Phosphatidylethanolamine (734 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Presenilin-1 (52 kDa, Protein from Homo sapiens)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Gamma-secretase subunit aph-1a (29 kDa, Protein from Homo sapiens)
- Human gamma-secretase (Complex from Homo sapiens)
- ~{tert}-butyl ~{n}-[(2~{s},3~{r},5~{r})-6-[[(2~{s})-1-[[(2~{s})-1-azanyl-1-oxidanylidene-3-phenyl-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-3-oxidanyl-6-oxidanylidene-1-phenyl-5-(phenylmethyl)hexan-2-yl]carbamate (672 Da, Ligand)
- 1-[(1s)-1-(4-fluorophenyl)ethyl]-3-[[3-methoxy-4-(4-methylimidazol-1-yl)phenyl]methylidene]piperidin-2-one (419 Da, Ligand)
- Gamma-secretase subunit pen-2 (16 kDa, Protein from Homo sapiens)
- Nicastrin (78 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Recognition of the Amyloid Precursor Protein by Human gamma-secretase
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 6iyc
Q-score: 0.567
Zhou R, Yang G, Guo X, Zhou Q, Lei J, Shi Y
Science (2019) 363 [ DOI: doi:10.1126/science.aaw0930 Pubmed: 30630874 ]
- Cholesterol (386 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Gamma-secretase subunit aph-1a (29 kDa, Protein from Homo sapiens)
- Human gamma-secretase (Complex from Homo sapiens)
- Gamma-secretase subunit pen-2 (12 kDa, Protein from Homo sapiens)
- Amyloid-beta a4 protein (11 kDa, Protein from Homo sapiens)
- Presenilin-1 (52 kDa, Protein from Homo sapiens)
- N-acetyl-d-glucosamine (221 Da, Ligand)
- Nicastrin (78 kDa, Protein from Homo sapiens)
- Beta-d-mannose (180 Da, Ligand)
Cryo-EM structure of gamma secretase in complex with a Notch fragment
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6idf
Q-score: 0.545
Yang G, Zhou R, Zhou Q, Guo X, Yan C, Ke M, Lei J, Shi Y
Nature (2019) 565 pp. 192-197 [ Pubmed: 30598546 DOI: doi:10.1038/s41586-018-0813-8 ]
- Cholesterol (386 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Gamma-secretase subunit aph-1a (29 kDa, Protein from Homo sapiens)
- Gamma-secretase subunit pen-2 (12 kDa, Protein from Homo sapiens)
- Presenilin-1 (52 kDa, Protein from Homo sapiens)
- Gamma-secretase (Complex from Homo sapiens)
- Notch1 (14 kDa, Protein from Homo sapiens)
- N-acetyl-d-glucosamine (221 Da, Ligand)
- Nicastrin (78 kDa, Protein from Homo sapiens)
- Beta-d-mannose (180 Da, Ligand)
Negative stain electron microscopy structure of native human Presenilin 1 (PS1) complex
Li Y, Lu SH, Tsai CJ, Bohm C, Qamar S, Dodd RB, Meadows W, Jeon A, McLeod A, Chen F, Arimon M, Berezovska O, Hyman BT, Tomita T, Iwatsubo T, Johnson CM, Farrer LA, Schmitt-Ulms G, Fraser PE, St George-Hyslop PH
Structure (2014) 22 pp. 125-135 [ Pubmed: 24210759 DOI: doi:10.1016/j.str.2013.09.018 ]
- Gamma-secretase subunit aph-1a (Protein from Homo sapiens)
- Gamma-secretase subunit pen-2 (Protein from Homo sapiens)
- Native human presenilin 1 (ps1) complex (200 kDa, Sample)
- Nicastrin (Protein from Homo sapiens)
- Presenilin-1 (Protein from Homo sapiens)
Negative stain electron microscopy structure of compound E-bound human Presenilin 1 (PS1) complex
Li Y, Lu SH, Tsai CJ, Bohm C, Qamar S, Dodd RB, Meadows W, Jeon A, McLeod A, Chen F, Arimon M, Berezovska O, Hyman BT, Tomita T, Iwatsubo T, Johnson CM, Farrer LA, Schmitt-Ulms G, Fraser PE, St George-Hyslop PH
Structure (2014) 22 pp. 125-135 [ Pubmed: 24210759 DOI: doi:10.1016/j.str.2013.09.018 ]
- Gamma-secretase subunit aph-1a (Protein from Homo sapiens)
- E ((s,s)- 2-[2-(3,5-difluorophenyl)-acetylamino]-n-(1-methyl-2-oxo-5-phenyl-2,3-dihydro-1h-benzo[e][1,4]diazepin-3-yl)-propionamide) (Ligand from synthetic construct)
- Gamma-secretase subunit pen-2 (Protein from Homo sapiens)
- Compound e-bound human presenilin 1 (ps1) complex (200 kDa, Sample)
- Nicastrin (Protein from Homo sapiens)
- Presenilin-1 (Protein from Homo sapiens)